チュートリアル講演 Design Automation for Digital and Flow-based Microfluidic Biochips
- 2017年3月7日（火） 16:00–17:00
立命館大学 びわこくさつキャンパス ローム記念館 ５階会議室１
滋賀県 草津市 野路東１丁目１－１
16:00–17:00 Design Automation for Digital and Flow-based Microfluidic Biochips
- During last two decades, an emerging technology of Lab-on-a-Chips (LOCs) or Biochips has been studied by the researchers of interdisciplinary fields to develop microfluidic chips that can implement wide-range of biochemical laboratory test protocols (a.k.a. bioassays). A marriage of microelectronics and in-vitro diagnostics areas has led to this field of interdisciplinary research around LOCs. In contrast to continuous-flow microfluidic chips, digital microfluidic (DMF) biochips are popular microfluidic LOCs that can implement bioassays on an electrode array of a few square centimeters in size by manipulating micro/nano/pico liter volume fluid droplets. The functionality of a DMF biochip includes the following operations: dispensing the desired amount of fluids to the chip from the outside world as droplets, transporting the droplets on-chip to appropriate locations, mixing and splitting of several droplets, executing a well-defined bioassay on a chip, and finally analyzing the results at an on-chip detection site. Recently, computer-aided-design (CAD) techniques have been extensively used for developing DMF biochips. It is expected that in near future, with the help of CAD techniques, tube and pipette based biochemical laboratory protocols will be revolutionized as small size biochips as the valve and tube based technology for electronic devices has been shifted to today’s semiconductor based very-large-scale-integration (VLSI) chips. Dr. Sudip Roy’s research envisions the computer-aided-design (CAD) research to develop DMF biochips by designing algorithms for automated sample preparation (dilution and mixing) on such chips. Mixing and dilution of fluids are fundamental preprocessing steps in almost all biochemical laboratory protocols. Mixing of two or more fluids with a given ratio is often required as a preprocessing step of many real-life biochemical protocols, e.g., polymerase chain reaction (PCR). Dilution of a biochemical fluid is the special case of mixing, where only two different types of fluids, one of which is a buffer solution, are mixed at a certain ratio corresponding to the desired concentration. The dilution is commonly used in biological studies to create a variety of concentrations of the stock solution by mixing it with its diluents and it is required for sample preparation in many bioassays, e.g., real-time PCR, immunoassays, etc. For high-throughput applications, it is a challenge to determine the sequence of minimum number of mix-split steps for on-chip sample preparation. Furthermore, the production of waste droplets and/or the reactant fluid usage should be minimized. Moreover, design automation tools are necessary for optimizing the layout of the biochips. In his talk, he will provide an easy tutorial on design automation issues for both digital and flow-based microfluidic biochips. He will also discuss about some computer-aided-design (CAD) techniques for automated and on-chip fluidic sample preparation (dilution and mixing) of biochemical fluids using microfluidic biochips.
Dr. Sudip Roy